Calculator
DNP amine
A carbonyl-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent carbonyl-reactive FRET quencher paired with Trp or Tyr.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DNP amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 442.106 µL | 2.211 mL | 4.421 mL | 22.105 mL | 44.211 mL |
| 5 mM | 88.421 µL | 442.106 µL | 884.212 µL | 4.421 mL | 8.842 mL |
| 10 mM | 44.211 µL | 221.053 µL | 442.106 µL | 2.211 mL | 4.421 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) | Correction Factor (260 nm) |
| ICG amine | 789 | 813 | 230000 | 0.076 | - |
| XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.11 | 0.30 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.56 | 0.43 |
| XFD532 amine | 534 | 553 | 81000 | 0.09 | 0.24 |
| XFD555 amine | 553 | 568 | 155000 | 0.08 | 0.08 |
| XFD568 amine | 579 | 603 | 91300 | 0.46 | 0.45 |
| XFD647 amine | 650 | 671 | 270000 | 0.03 | - |
| XFD750 amine | 752 | 776 | 290000 | 0.04 | - |
| XFD350 amine | 343 | 441 | 19000 | 0.19 | 0.25 |
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References
View all 124 references: Citation Explorer
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Evaluation of an imaging platform during the development of a FRET protease assay
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
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