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Dihydrorhodamine 123 *CAS 109244-58-8*
Dihydrorhodamine 123 is by far the most-used probe for measurement of intracellular H2O2. DHR 123 is oxidized directly to rhodamine 123, which is excitable at 488 and emits at 515 nm in the same emission range as FITC. It is widely used in human neutrophils, human eosinophils, HL60 cells, rat mast cells, guinea pig neutrophils, cultured chondrocytes, rat brain, rat renal proximal tubular cells, mesangial cells and L929 cells. In combination with other fluorescent reagents (such as surface receptor analysis by fluorescent antibodies, cell viability using propidium iodide, and calcium indicators) this probe can be used for multiplex measurements.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrorhodamine 123 *CAS 109244-58-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 288.7 µL | 1.444 mL | 2.887 mL | 14.435 mL | 28.87 mL |
| 5 mM | 57.74 µL | 288.7 µL | 577.401 µL | 2.887 mL | 5.774 mL |
| 10 mM | 28.87 µL | 144.35 µL | 288.7 µL | 1.444 mL | 2.887 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 |
Citations
View all 8 citations: Citation Explorer
Variations in reactive oxygen species generation by urban airborne particulate matter in lung epithelial cells—impact of inorganic fraction
Authors: Mazuryk, Olga and Stochel, Grazyna and Brindell, Ma{\l}gorzata
Journal: Frontiers in chemistry (2020): 581752
Authors: Mazuryk, Olga and Stochel, Grazyna and Brindell, Ma{\l}gorzata
Journal: Frontiers in chemistry (2020): 581752
Direct macromolecular drug delivery to cerebral ischemia area using neutrophil-mediated nanoparticles
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Journal: Theranostics (2017): 3260
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Journal: Theranostics (2017): 3260
Liver necrosis and lethal systemic inflammation in a murine model of Rickettsia typhi infection: role of neutrophils, macrophages and NK cells
Authors: Papp, Stefanie and Moderzynski, Kristin and Rauch, Jessica and Heine, Liza and Kuehl, Svenja and Richardt, Ulricke and Mueller, Heidelinde and Fleischer, Bernhard and Osterloh, Anke
Journal: PLoS Negl Trop Dis (2016): e0004935
Authors: Papp, Stefanie and Moderzynski, Kristin and Rauch, Jessica and Heine, Liza and Kuehl, Svenja and Richardt, Ulricke and Mueller, Heidelinde and Fleischer, Bernhard and Osterloh, Anke
Journal: PLoS Negl Trop Dis (2016): e0004935
References
View all 20 references: Citation Explorer
Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD
Authors: Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.
Journal: Clin Chem (2007): 890
Authors: Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.
Journal: Clin Chem (2007): 890
Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals
Authors: Wrona M, Patel K, Wardman P.
Journal: Free Radic Biol Med (2005): 262
Authors: Wrona M, Patel K, Wardman P.
Journal: Free Radic Biol Med (2005): 262
Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123
Authors: Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.
Journal: J Fluoresc (2004): 17
Authors: Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.
Journal: J Fluoresc (2004): 17
Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay
Authors: Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.
Journal: J Allergy Clin Immunol (2003): 374
Authors: Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.
Journal: J Allergy Clin Immunol (2003): 374
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
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