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DEAC [7-Diethylaminocoumarin-3-carboxylic acid] *CAS 50995-74-9*
7-Diethylaminocoumarin-3-carboxylic acid can be used to create blue-fluorescent bioconjugates. It has quite strong blue fluorescence. When compared with AMCA conjugates, conjugates of the UV-light-excitable 7-dialkylaminocoumarin fluorophore have much longer-wavelength excitation and slightly longer-wavelength emission spectra (~470 nm).
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DEAC [7-Diethylaminocoumarin-3-carboxylic acid] *CAS 50995-74-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 382.746 µL | 1.914 mL | 3.827 mL | 19.137 mL | 38.275 mL |
| 5 mM | 76.549 µL | 382.746 µL | 765.492 µL | 3.827 mL | 7.655 mL |
| 10 mM | 38.275 µL | 191.373 µL | 382.746 µL | 1.914 mL | 3.827 mL |
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References
View all 4 references: Citation Explorer
Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146
A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562
Glutamate 779, an intramembrane carboxyl, is essential for monovalent cation binding by the Na,K-ATPase
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892
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