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Dansyl-X SE has higher labeling efficiency and better stability than Dansyl chloride. Dansyl dyes have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl dyes an important tool for biophysical studies. They are particularly useful for preparing fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes or other biological receptors. Dansyl protein conjugates have fluorescence lifetimes of 10-20 nanoseconds.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Dansyl-X, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 216.671 µL | 1.083 mL | 2.167 mL | 10.834 mL | 21.667 mL |
| 5 mM | 43.334 µL | 216.671 µL | 433.341 µL | 2.167 mL | 4.333 mL |
| 10 mM | 21.667 µL | 108.335 µL | 216.671 µL | 1.083 mL | 2.167 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| Dansyl-X, acid | 335 | 518 | 3300 | 0.387 |
References
View all 33 references: Citation Explorer
Phenylisothiocyanate and dansyl chloride precolumn derivatizations for the high-performance liquid chromatography analysis of the antitumoral agent ES-285 in dog plasma
Authors: Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1
Authors: Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1
Dansyl chloride derivatization of methamphetamine: a method with advantages for screening and analysis of methamphetamine in urine
Authors: Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.
Journal: J Anal Toxicol (2002): 17
Authors: Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.
Journal: J Anal Toxicol (2002): 17
Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride
Authors: Naassner M, Mergler M, Wolf K, Schuphan I.
Journal: J Chromatogr A (2002): 133
Authors: Naassner M, Mergler M, Wolf K, Schuphan I.
Journal: J Chromatogr A (2002): 133
Automated pre-column derivatization of amines in biological samples with dansyl chloride and with or without post-column chemiluminescence formation by using TCPO-H2O2
Authors: Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.
Journal: Analyst (1998): 2871
Authors: Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.
Journal: Analyst (1998): 2871
A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence
Authors: Yamada H, Ikeda-Wada S, Oguri K.
Journal: Biol Pharm Bull (1998): 778
Authors: Yamada H, Ikeda-Wada S, Oguri K.
Journal: Biol Pharm Bull (1998): 778
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