Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 544 |
Emission (nm) | 567 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence* |
Overview | ![]() ![]() |
Excitation (nm) 544 | Emission (nm) 567 |
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 575/26 nm filter |
Instrument specification(s) | PE channel |
Fluorescence microscope
Excitation | Cy3/TRITC filter set |
Emission | Cy3/TRITC filter set |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Treat samples as desired
Prepare and add Xite™ Red beta-D-galactopyranoside working solution to samples
Incubate samples at 37 °C for 15 to 45 minutes
Monitor the fluorescence intensity with fluorescence microscope using Cy3/TRITC filter set or flow cytometer using 575/26 nm filter (PE channel)
To ensure accurate results, allow all kit components to reach room temperature before beginning the experiment.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 µL of DMSO (Component C) into Xite™ Red beta-D-galactopyranoside (Component A) and mix thoroughly.
Note: Divide any remaining Xite™ Red beta-D-galactopyranoside stock solution into individual single-use aliquots. Store these aliquots at -20°C, protected from light.
PREPARATION OF WORKING SOLUTION
To prepare the Xite™ Red beta-D-galactopyranoside working solution, mix 10 µL of the Xite™ Red beta-D-galactopyranoside stock solution with 1 mL of Assay Buffer.
Note: Xite™ Red beta-D-galactopyranoside working solution should be used promptly.
SAMPLE EXPERIMENTAL PROTOCOL
Treat your samples as desired.
Wash the cells with a buffer of your choice such as DPBS.
Note: For selectively tracking β-Gal in live cells, cells can be treated with Bafilomycin A1 for blocking endogenous β-Gal. The optimum concentration of Bafilomycin A1 may vary on the type of cells.
Add 100 µL of Xite™ Red beta-D-galactopyranoside working solution, and incubate the samples at 37 °C incubator for 15 to 45 minutes.
Note: The optimal time for incubation needs to be determined carefully.
Remove the working solution and wash cells with buffer of your choice.
Resuspend the cells in the Assay Buffer (Component B) and monitor the fluorescence intensity with a flow cytometer using a 575/26 nm filter (PE channel) or fluorescence microscope using a Cy3/TRITC filter set.
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Images
![Fixability test with Cell Meter™ Cellular Senescence Activity Assay Kit using a NovoCyte Flow Cytometer (ACEA Biosciences). 9L-LacZ cells were incubated with DMSO or Xite™ Red beta-D-galactopyranoside for 45 mins at 37 °C. The signal before and after fixation was acquired using PE channel. (Cells were then fixed with 4% formaldehyde for 20 minutes at room temperature, and wash once.) ](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-cellular-senescence-activity-assay-kit-red-fluorescence%2Ffigure-for-cell-meter-cellular-senescence-activity-assay-kit-red-fluorescence_zRxFB.jpg&w=3840&q=75)
Citations
Authors: Scaramuzza, Shaun and Jones, Rebecca M and Sadurni, Martina Muste and Reynolds-Winczura, Alicja and Poovathumkadavil, Divyasree and Farrell, Abigail and Natsume, Toyoaki and Rojas, Patricia and Cuesta, Cyntia Fernandez and Kanemaki, Masato T and others,
Journal: Nature Communications (2023): 5071
Authors: Park, Chun Shik and Yoshihara, Hiroki and Gao, Qingsong and Qu, Chunxu and Iacobucci, Ilaria and Ghate, Pankaj S and Connelly, Jon P and Pruett-Miller, Shondra M and Wagner, Ben and Robinson, Camenzind G and others,
Journal: Cell Reports (2023): 112804
Authors: Pham, Alexander M and Ortiz, Luz E and Lukacher, Aron E and Kwun, Hyun Jin
Journal: Cells (2023): 380
References
Authors: Saito, Yuki and Chikenji, Takako S and Matsumura, Takashi and Nakano, Masako and Fujimiya, Mineko
Journal: Nature communications (2020): 889
Authors: Pan, Xiao-Xi and Wu, Fang and Chen, Xiao-Hui and Chen, Dong-Rui and Chen, Hong-Jin and Kong, Ling-Ran and Ruan, Cheng-Chao and Gao, Ping-Jin
Journal: Cardiovascular research (2020)
Authors: Rani, Mohammad Hasanuzzaman and Liu, Qunen and Yu, Ning and Zhang, Yingxin and Wang, Beifang and Cao, Yongrun and Zhang, Yue and Islam, Md Anowerul and Zegeye, Workie Anley and Cao, Liyong and Cheng, Shihua
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Authors: Tang, Yan-Long and Zhang, Cheng-Gui and Liu, Heng and Zhou, Yue and Wang, Ya-Ping and Li, Yuan and Han, Yan-Jun and Wang, Cui-Li
Journal: Medical science monitor : international medical journal of experimental and clinical research (2020): e918207
Authors: Lü, Guohua and Li, Lei and Wang, Bing and Kuang, Lei
Journal: Aging (2020)
Authors: Han, Xiaojuan and Chen, Honghan and Gong, Hui and Tang, Xiaoqiang and Huang, Ning and Xu, Weitong and Tai, Haoran and Zhang, Gongchang and Zhao, Tingting and Gong, Chuhui and Wang, Shuang and Yang, Yu and Xiao, Hengyi
Journal: The Journal of biological chemistry (2020)
Authors: Metur, Shree Padma and Klionsky, Daniel J
Journal: Autophagy (2020): 389-390
Authors: Yang, Yuyu and Li, Zilong and Guo, Junli and Xu, Yong
Journal: Biochimica et biophysica acta. Molecular basis of disease (2020): 165723
Authors: Xu, Cheng Lin and Sang, Ben and Liu, Guang Zhi and Li, Jin Ming and Zhang, Xu Dong and Liu, Lian Xin and Thorne, Rick F and Wu, Mian
Journal: Nucleic acids research (2020)
Authors: Aquino-Martinez, Ruben and Rowsey, Jennifer L and Fraser, Daniel G and Eckhardt, Brittany A and Khosla, Sundeep and Farr, Joshua N and Monroe, David G
Journal: Bone (2020): 115220
Application notes
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