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Biotinylated goat anti-mouse IgG (H+L)

This biotinylated goat anti-mouse IgG (H+L) is affinity-purified to have high affinity. It recognizes both heavy and light chains (H+L). It is biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. It can be used for tissue and cell staining, in situ hybridization, Elispot ELISAs and blots.

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
FITC goat anti-mouse IgG (H+L)491516730000.920.35-
Cy3® goat anti-mouse IgG (H+L)55556915000010.1510.0730.07
Cy5® goat anti-mouse IgG (H+L)65167025000010.271, 0.420.030.02
Cy7® goat anti-mouse IgG (H+L)7567792500000.30.0360.05
FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*491516730000.920.35-
Cy3® goat anti-mouse IgG (H+L) *Cross Adsorbed*55556915000010.1510.0730.07
Cy5® goat anti-mouse IgG (H+L) *Cross Adsorbed*65167025000010.271, 0.420.030.02
Cy7® goat anti-mouse IgG (H+L) *Cross Adsorbed*7567792500000.30.0360.05
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.190.25
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.110.3
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.560.43
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Citations

View all 5 citations: Citation Explorer
Virtual crossmatching reveals upregulation of placental HLA-Class II in chronic histiocytic intervillositis
Authors: Brady, Chloe A and Ford, Laura B and Moss, Chloe and Zou, Zhiyong and Crocker, Ian P and Heazell, Alexander EP
Journal: Scientific Reports (2024): 1--14
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo
Journal: Tumor Biology (2016): 1--12
Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: Shi, W and Huang, CJ and Xu, XD and Jin, GH and Huang, RQ and Huang, JF and Chen, YN and Ju, SQ and Wang, Y and Shi, YW and others, undefined
Journal: Acta Biomaterialia (2016): 247--261
The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Huang, Chuanjun and Zhao, Longxiang and Gu, Jun and Nie, Dekang and Chen, Yinan and Zuo, Hao and Huan, Wei and Shi, Jinlong and Chen, Jian and Shi, Wei
Journal: Biomedical Materials (2016): 035004

References

View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Page updated on November 21, 2024

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Catalog Number16729
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Physical properties

Molecular weight

~150000

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Image comparison of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT's biotinylated goat anti-mouse IgG (H&amp;L) (Left, Cat# 16729) or Jackson's biotinylated goat anti-mouse IgG conjugate (Right). At the end, cells were incubated with AAT's iFluor 488-streptavidin conjugate (Cat# 16955).
Image comparison of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT's biotinylated goat anti-mouse IgG (H&amp;L) (Left, Cat# 16729) or Jackson's biotinylated goat anti-mouse IgG conjugate (Right). At the end, cells were incubated with AAT's iFluor 488-streptavidin conjugate (Cat# 16955).
Image comparison of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT's biotinylated goat anti-mouse IgG (H&amp;L) (Left, Cat# 16729) or Jackson's biotinylated goat anti-mouse IgG conjugate (Right). At the end, cells were incubated with AAT's iFluor 488-streptavidin conjugate (Cat# 16955).