AMP-Fluorescein conjugate calibrator

AMP-Fluorescein conjugate calibrator is desigend to use with our green fluorescent FAM-cAMP PDE IV substrate (#Cat: 13602), a cAMP derivative that is a specific substrate for phosphodiesterase (PDE) IV. This AMP-Fluorescein conjugate calibrator is the hydrolyzed product of FAM-cAMP PDE IV substrate by PDE IV. It is used to calibrate the hydrolysis rate of FAM-cAMP PDE IV substrate by PDE IV.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
GMP-Fluorescein conjugate calibrator	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

References

View all 34 references: Citation Explorer

Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide phosphodiesterase 3B (Pde3b) gene in differentiating 3T3-L1 preadipocytes
Authors: Liu H, Tang JR, Choi YH, Napolitano M, Hockman S, Taira M, Degerman E, Manganiello VC.
Journal: J Biol Chem (2006): 21096

Immunohistochemical distribution of cAMP- and cGMP-Phosphodiesterase (PDE) isoenzymes in the human prostate
Authors: Sampaio FJ., undefined
Journal: Int Braz J Urol (2006): 368

Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5
Authors: Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, Houslay MD.
Journal: Biochem J (2006): 23

cAMP phosphodiesterase-4A1 (PDE4A1) has provided the paradigm for the intracellular targeting of phosphodiesterases, a process that underpins compartmentalized cAMP signalling
Authors: Huston E, Houslay TM, Baillie GS, Houslay MD.
Journal: Biochem Soc Trans (2006): 504

Expression and Activity of cAMP Phosphodiesterase Isoforms in Pulmonary Artery Smooth Muscle Cells from Patients with Pulmonary Hypertension: Role for PDE1
Authors: Murray F, Patel HH, Suda RY, Zhang S, Thistlethwaite P, Yuan JX, Insel PA.
Journal: Am J Physiol Lung Cell Mol Physiol. (2006)

Page updated on November 21, 2024

Ordering information

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Catalog Number	13610
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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
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Physical properties

Molecular weight	~800
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200