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Figure 1. AF488 NHS Ester is the same molecule to the Alexa Fluor® 488 NHS Ester (#A20000 and A20100 from ThermoFisher, Alexa Fluor® is the trademark of ThermoFisher). Although the Alexa Fluor® 488 NHS Ester is one of the most common green fluorescent labeling dyes, its purity has been quite challenging. ThermoFisher provides it with purity of ~50-60% while a few other vendors offer it at even lower purities. Among all the impurities, we noted that the Bis-NHS ester of AF488 is quite detrimental since it could give a non-fluorescent product. This high purity AF488 NHS ester (#1080) of AAT Bioquest is free of the detrimental AF488 Bis-NHS ester impurity with much higher purity (top graph).
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