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NBD-X, succinimidyl ester *CAS 145195-58-0*
Compared to NBD chloride and fluoride, NBD-X acid gives better yield for labeling biopolymers. The fluorescence spectra of NBD derivatives are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-X, succinimidyl ester *CAS 145195-58-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 255.539 µL | 1.278 mL | 2.555 mL | 12.777 mL | 25.554 mL |
| 5 mM | 51.108 µL | 255.539 µL | 511.078 µL | 2.555 mL | 5.111 mL |
| 10 mM | 25.554 µL | 127.769 µL | 255.539 µL | 1.278 mL | 2.555 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 2 citations: Citation Explorer
Comparison among different “revealers” in the study of accelerated blood clearance phenomenon
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
References
View all 102 references: Citation Explorer
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Journal: Analyst (2006): 236
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Two novel missense and one novel nonsense CFTR mutations in Iranian males with congenital bilateral absence of the vas deferens
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Journal: Mol Hum Reprod (2006): 717
Authors: Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.
Journal: Mol Hum Reprod (2006): 717
In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer
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Journal: Embo J (2006): 4728
Authors: Mense M, Vergani P, White DM, Altberg G, Nairn AC, Gadsby DC.
Journal: Embo J (2006): 4728
Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
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Authors: Higashi Y, Nakamura S, Matsumura H, Fujii Y.
Journal: Biomed Chromatogr (2006): 423
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