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7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*
7-Methoxycoumarin-3-carboxylic acid is used for HPLC derivatization. It is also used to develop FRET probes for analyzing protease activities.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 454.174 µL | 2.271 mL | 4.542 mL | 22.709 mL | 45.417 mL |
| 5 mM | 90.835 µL | 454.174 µL | 908.348 µL | 4.542 mL | 9.083 mL |
| 10 mM | 45.417 µL | 227.087 µL | 454.174 µL | 2.271 mL | 4.542 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
| 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* | 352 | 407 | 0.0815 |
References
View all 3 references: Citation Explorer
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
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