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7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1*
7-Hydroxycoumarin-3-carboxylic acid is a blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester catalyzed by EDAC.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 485.084 µL | 2.425 mL | 4.851 mL | 24.254 mL | 48.508 mL |
| 5 mM | 97.017 µL | 485.084 µL | 970.167 µL | 4.851 mL | 9.702 mL |
| 10 mM | 48.508 µL | 242.542 µL | 485.084 µL | 2.425 mL | 4.851 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
| 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* | 355 | 405 | 0.171 |
References
View all 16 references: Citation Explorer
In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Flow injection analysis of binding reaction between fluorescent lectin and cells
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
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