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7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*

This compound is used as a calibration standard for 7-hydroxy-4-methylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM567.634 µL2.838 mL5.676 mL28.382 mL56.763 mL
5 mM113.527 µL567.634 µL1.135 mL5.676 mL11.353 mL
10 mM56.763 µL283.817 µL567.634 µL2.838 mL5.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 19 references: Citation Explorer
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Page updated on October 31, 2024

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Physical properties

Molecular weight

176.17

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.1

Extinction coefficient (cm -1 M -1)

18000

Excitation (nm)

360

Emission (nm)

448

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

90-33-5

Platform

Fluorescence microplate reader

Recommended plateBlack wall, clear bottom
Instrument specification(s)Bottom read mode
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