6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] CAS 174568-68-4

Tetramethylrhodamine-6-maleimide is the other purified single isomer of tetramethylrhodamine-5 (and 6)-maleimide mixed isomers. Compared to the 5-isomer, tetramethylrhodamine-6-maleimide is preferably used for thiol modifications of nucleotides and nucleic acids.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	207.68 µL	1.038 mL	2.077 mL	10.384 mL	20.768 mL
5 mM	41.536 µL	207.68 µL	415.36 µL	2.077 mL	4.154 mL
10 mM	20.768 µL	103.84 µL	207.68 µL	1.038 mL	2.077 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.32	0.178
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] CAS 154480-30-5	552	578	90000	0.32	0.178
6-TAMRA cadaverine	552	578	90000	0.32	0.178
6-TAMRA ethylenediamine	552	578	90000	0.32	0.178
6-TAMRA azide	552	578	90000	0.32	0.178
6-TAMRA alkyne	552	578	90000	0.32	0.178
6-TAMRA CPG 1000 Å	552	578	90000	0.32	0.178
6-NED maleimide	545	567	74000¹	-	-

References

View all 21 references: Citation Explorer

Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533

Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)

4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)

Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338

The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505

Page updated on November 20, 2024

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Physical properties

Molecular weight	481.51
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	174568-68-4