6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8

Name: 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*
Brand: AAT Bioquest
SKU: 116
Availability: InStock

6-FAM, SE is the amine-reactive succinimidyl ester of single isomer 6-FAM acid. It is one of the most popular green fluorescent reagents used for labeling nucleotides and nucleic acids. Compared to 5-FAM, 6-FAM is less often used to prepare small molecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	211.242 µL	1.056 mL	2.112 mL	10.562 mL	21.124 mL
5 mM	42.248 µL	211.242 µL	422.485 µL	2.112 mL	4.225 mL
10 mM	21.124 µL	105.621 µL	211.242 µL	1.056 mL	2.112 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-FAM [5-(and-6)-Carboxyfluorescein] CAS 72088-94-9	493	517	83000	0.32	0.178
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] CAS 92557-80-7	493	517	83000	0.32	0.178
6-FAM Azide	493	517	83000	0.32	0.178
6-FAM Alkyne	493	517	83000	0.32	0.178
6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] CAS 204697-37-0	493	517	83000	0.32	0.178
6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] Single Isomer	533	559	73000	-	0.15
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	-	-
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	-	0.13
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.24	0.214
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.32	0.178
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] CAS#: 216699-36-4	578	604	82000	-	0.168
6-ROXtra™ SE Superior 6-ROX Replacement	578	595	-	-	-
6-VIC, SE [6-VIC NHS ester]	526	543	-	-	-
6-NED, SE [6-NED NHS ester]	545	567	74000¹	-	-
Show More (5)

Citations

View all 3 citations: Citation Explorer

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 137 references: Citation Explorer

Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80

Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165

Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379

Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262

Page updated on December 3, 2024

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Catalog Number	116117118
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Physical properties

Molecular weight	473.39
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	92557-81-8