5-Propargylamino-3'-azidomethyl-dUTP
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 576.24 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Dideoxynucleotides (ddNTPs), DNA Methylation, Fluorescent in situ hybridization (FISH), Next Generation Sequencing (NGS), Nucleic Acid Building Blocks, RNA Purification & Analysis, Sanger Sequencing
CAS 666847-57-0 | Molecular weight 576.24 |
5-Propargylamino-3'-azidomethyl-dUTP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS). NGS uses a similar chain termination method to the earlier Sanger sequencing, but NGS is carried out by fluorescence-labeled nucleotide analogs acting as reversible terminators of the amplification reaction. NGS relies on the blockade of DNA polymerization that is reversible while the Sanger sequencing uses the irreversible blockade of DNA polymerization by ddNTPs. Another different feature of NGS is that the clonal amplification in vitro to multiply the number of molecules to be sequenced is conducted by means of bridge PCR. In this platform, the fragments are joined to primers immobilized on a solid surface, performing an amplification in situ, generating clusters of DNA with identical molecules. In each cycle, the four nucleotides of reversible termination are simultaneously added and incorporated by the polymerase they complement. These nucleotides are chemically blocked—by substituting the 3′-OH group for a 3′-o-azidomethyl group—to prevent the polymerase from incorporating more than one nucleotide in each cycle. Upon incorporation of a nucleotide, a fluorescence signal is measured in different channels for different bases. Concerning the next cycle, the nucleotides that have not been incorporated are washed and the chemical blockade of the 3′ end is removed with TCEP. Once the fluorescence signal is collected, a new cycle begins, repeating this dynamic until the sequencing of each fragment is finished. In summary, the NGS sequencing reaction is carried out in three steps: addition of nucleotides, imaging, and regeneration of 3′-OH by fluorophore cleavage.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of 5-Propargylamino-3'-azidomethyl-dUTP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 173.539 µL | 867.694 µL | 1.735 mL | 8.677 mL | 17.354 mL |
5 mM | 34.708 µL | 173.539 µL | 347.078 µL | 1.735 mL | 3.471 mL |
10 mM | 17.354 µL | 86.769 µL | 173.539 µL | 867.694 µL | 1.735 mL |
Molarity calculator
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References
View all 50 references: Citation Explorer
Current scenario of the genetic testing for rare neurological disorders exploiting next generation sequencing.
Authors: Di Resta, Chiara and Pipitone, Giovanni Battista and Carrera, Paola and Ferrari, Maurizio
Journal: Neural regeneration research (2021): 475-481
Authors: Di Resta, Chiara and Pipitone, Giovanni Battista and Carrera, Paola and Ferrari, Maurizio
Journal: Neural regeneration research (2021): 475-481
Application of Next Generation Sequencing in Laboratory Medicine.
Authors: Zhong, Yiming and Xu, Feng and Wu, Jinhua and Schubert, Jeffrey and Li, Marilyn M
Journal: Annals of laboratory medicine (2021): 25-43
Authors: Zhong, Yiming and Xu, Feng and Wu, Jinhua and Schubert, Jeffrey and Li, Marilyn M
Journal: Annals of laboratory medicine (2021): 25-43
Factor XIII deficiency in two Spanish families with a novel variant in gene F13A1 detected by next-generation sequencing; symptoms and clinical management.
Authors: Moret, Andrés and Zúñiga, Ángel and Ayala, Javier Marco and Liquori, Alessandro and Cid, Ana Rosa and Haya, Saturnino and Ferrando, Fernando and Blanquer, Amando and Cervera, José and Bonanad, Santiago
Journal: Journal of thrombosis and thrombolysis (2020): 686-688
Authors: Moret, Andrés and Zúñiga, Ángel and Ayala, Javier Marco and Liquori, Alessandro and Cid, Ana Rosa and Haya, Saturnino and Ferrando, Fernando and Blanquer, Amando and Cervera, José and Bonanad, Santiago
Journal: Journal of thrombosis and thrombolysis (2020): 686-688
Genomic Study of Chinese Quadruple-negative GISTs Using Next-generation Sequencing Technology.
Authors: Wang, Si and Sun, Rui-Ze and Han, Qiang and Wang, Si-Yao and Wang, En-Hua and Liu, Yang
Journal: Applied immunohistochemistry & molecular morphology : AIMM (2020)
Authors: Wang, Si and Sun, Rui-Ze and Han, Qiang and Wang, Si-Yao and Wang, En-Hua and Liu, Yang
Journal: Applied immunohistochemistry & molecular morphology : AIMM (2020)
Genomic characterization of MICA gene using multiple next generation sequencing platforms: A validation study.
Authors: Zou, Yizhou and Duke, Jamie L and Ferriola, Deborah and Luo, Qizhi and Wasserman, Jenna and Mosbruger, Timothy L and Luo, Weiguang and Cai, Liang and Zou, Kevin and Tairis, Nikolaos and Damianos, Georgios and Pagkrati, Ioanna and Kukuruga, Debra and Huang, Yanping and Monos, Dimitri S
Journal: HLA (2020): 430-444
Authors: Zou, Yizhou and Duke, Jamie L and Ferriola, Deborah and Luo, Qizhi and Wasserman, Jenna and Mosbruger, Timothy L and Luo, Weiguang and Cai, Liang and Zou, Kevin and Tairis, Nikolaos and Damianos, Georgios and Pagkrati, Ioanna and Kukuruga, Debra and Huang, Yanping and Monos, Dimitri S
Journal: HLA (2020): 430-444
Next-generation sequencing in the diagnosis of viral encephalitis: sensitivity and clinical limitations.
Authors: Perlejewski, Karol and Bukowska-Ośko, Iwona and Rydzanicz, Małgorzata and Pawełczyk, Agnieszka and Caraballo Cortѐs, Kamila and Osuch, Sylwia and Paciorek, Marcin and Dzieciątkowski, Tomasz and Radkowski, Marek and Laskus, Tomasz
Journal: Scientific reports (2020): 16173
Authors: Perlejewski, Karol and Bukowska-Ośko, Iwona and Rydzanicz, Małgorzata and Pawełczyk, Agnieszka and Caraballo Cortѐs, Kamila and Osuch, Sylwia and Paciorek, Marcin and Dzieciątkowski, Tomasz and Radkowski, Marek and Laskus, Tomasz
Journal: Scientific reports (2020): 16173
Panel-based next-generation sequencing facilitates the characterization of childhood acute myeloid leukemia in clinical settings.
Authors: Ishida, Hisashi and Iguchi, Akihiro and Aoe, Michinori and Nishiuchi, Ritsuo and Matsubara, Takehiro and Keino, Dai and Sanada, Masashi and Shimada, Akira
Journal: Biomedical reports (2020): 46
Authors: Ishida, Hisashi and Iguchi, Akihiro and Aoe, Michinori and Nishiuchi, Ritsuo and Matsubara, Takehiro and Keino, Dai and Sanada, Masashi and Shimada, Akira
Journal: Biomedical reports (2020): 46
Impact of Sun Exposure and Tanning Patterns on Next-Generation Sequencing Mutations in Melanoma.
Authors: Loo, Kimberly and Soliman, Iman and Renzetti, Madelyn and Li, Tianyu and Wu, Hong and Reddy, Sanjay and Olszanski, Anthony J and Farma, Jeffrey M
Journal: The Journal of surgical research (2020): 147-153
Authors: Loo, Kimberly and Soliman, Iman and Renzetti, Madelyn and Li, Tianyu and Wu, Hong and Reddy, Sanjay and Olszanski, Anthony J and Farma, Jeffrey M
Journal: The Journal of surgical research (2020): 147-153
[The quality control of library construction for hybrid capture based next-generation sequencing using formalin-fixed and paraffin-embedded puncture samples].
Authors: Wang, W W and Xiao, L and Jiang, G Z and Shi, Y and Chen, G and Wu, X F and Huang, R F and Zhao, D H and Xu, W N and Yan, B and Liu, J J and Ye, J Y and Zhang, Z and Li, W C
Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (2020): 1064-1067
Authors: Wang, W W and Xiao, L and Jiang, G Z and Shi, Y and Chen, G and Wu, X F and Huang, R F and Zhao, D H and Xu, W N and Yan, B and Liu, J J and Ye, J Y and Zhang, Z and Li, W C
Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (2020): 1064-1067
Glioblastomas harboring gene fusions detected by next-generation sequencing.
Authors: Woo, Ha Young and Na, Kiyong and Yoo, Jihwan and Chang, Jong Hee and Park, Young Nyun and Shim, Hyo Sup and Kim, Se Hoon
Journal: Brain tumor pathology (2020): 136-144
Authors: Woo, Ha Young and Na, Kiyong and Yoo, Jihwan and Chang, Jong Hee and Park, Young Nyun and Shim, Hyo Sup and Kim, Se Hoon
Journal: Brain tumor pathology (2020): 136-144
Application notes
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Are mRNAs found in prokaryotes differ from those of eukaryotes?
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Are there any alternatives to Cy5?