5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*
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Physical properties
Molecular weight | 515.25 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Förster Resonance Energy Transfer (FRET), Kinases, Fluoresceins, Fluorescence Lifetime Imaging Microscopy (FLIM), Intracellular Zinc
CAS 63368-54-7 | Molecular weight 515.25 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein-5-maleimide. The resulted bioconjugates are quite stable.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 194.081 µL | 970.403 µL | 1.941 mL | 9.704 mL | 19.408 mL |
5 mM | 38.816 µL | 194.081 µL | 388.161 µL | 1.941 mL | 3.882 mL |
10 mM | 19.408 µL | 97.04 µL | 194.081 µL | 970.403 µL | 1.941 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluorescein.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluorescein.png&w=2048&q=50)
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.34 |
Show More (2) |
References
View all 10 references: Citation Explorer
Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015
Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75
Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726
Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726
Distances between 5-iodoacetamidofluorescein and the ATP and ouabain sites of (Na,K)-ATPase determined by fluorescence energy transfer
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197
5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2090
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2090
Steady-state fluorescence emission from the fluorescent probe, 5-iodoacetamidofluorescein, bound to hemoglobin
Authors: Hirsch RE, Zukin RS, Nagel RL.
Journal: Biochem Biophys Res Commun (1986): 489
Authors: Hirsch RE, Zukin RS, Nagel RL.
Journal: Biochem Biophys Res Commun (1986): 489
Ligand binding to (Na,K)-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2084
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2084
Fluorescent labeling of (Na+ + K+)-ATPase by 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: Biochim Biophys Acta (1982): 493
Authors: Kapakos JG, Steinberg M.
Journal: Biochim Biophys Acta (1982): 493
Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin
Authors: Wang YL, Taylor DL.
Journal: J Histochem Cytochem (1980): 1198
Authors: Wang YL, Taylor DL.
Journal: J Histochem Cytochem (1980): 1198
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
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