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5-dTMR [5-Carboxy-4,7-dichlorortetramethylrhodamine]
5-dTMR (5-Carboxy-4,7-dichlorortetramethylrhodamine) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dR6G and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dTMR [5-Carboxy-4,7-dichlorortetramethylrhodamine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 166.517 µL | 832.584 µL | 1.665 mL | 8.326 mL | 16.652 mL |
| 5 mM | 33.303 µL | 166.517 µL | 333.034 µL | 1.665 mL | 3.33 mL |
| 10 mM | 16.652 µL | 83.258 µL | 166.517 µL | 832.584 µL | 1.665 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
| 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.35 |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
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References
View all 6 references: Citation Explorer
Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7
Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5
Design strategies and performance of custom DNA sequencing primers
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4
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