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5-Carboxy-4'-aminomethylfluorescein

5-Carboxy-4'-aminomethylfluorescein is a key building block for developing Big Dye DNA sequencing terminators. It can also be incorporated into a peptide sequence to give fluoresceinated peptides.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxy-4'-aminomethylfluorescein to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM226.337 µL1.132 mL2.263 mL11.317 mL22.634 mL
5 mM45.267 µL226.337 µL452.673 µL2.263 mL4.527 mL
10 mM22.634 µL113.168 µL226.337 µL1.132 mL2.263 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)
6-Carboxy-4'-aminomethylfluorescein498525

References

View all 65 references: Citation Explorer
UV Raman spectroscopic study on TiO2. I. Phase transformation at the surface and in the bulk
Authors: Zhang J, Li M, Feng Z, Chen J, Li C.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 927
Genotypes of the cytochrome p450 isoform, CYP2C9, and the vitamin K epoxide reductase complex subunit 1 conjointly determine stable warfarin dose: a prospective study
Authors: Carlquist JF, Horne BD, Muhlestein JB, Lappe DL, Whiting BM, Kolek MJ, Clarke JL, James BC, Anderson JL.
Journal: J Thromb Thrombolysis. (2006)
Potentials of high-temperature anaerobic treatment and redox mediators for the reductive decolorization of azo dyes from textile wastewaters
Authors: Dos Santos AB, Cervantes FJ, van Lier JB.
Journal: Water Sci Technol (2006): 151
Investigation of the role of ENPP1 and TNAP genes in chondrocalcinosis
Authors: Zhang Y, Brown MA, Peach C, Russell G, Wordsworth BP.
Journal: Rheumatology (Oxford). (2006)
Intrahepatic cholestasis of pregnancy: three novel MDR3 gene mutations
Authors: Floreani A, Carderi I, Paternoster D, Soardo G, Azzaroli F, Esposito W, Variola A, Tommasi AM, Marchesoni D, Braghin C, Mazzella G.
Journal: Aliment Pharmacol Ther (2006): 1649
Page updated on December 17, 2024

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Physical properties

Molecular weight

441.82

Solvent

DMSO

Spectral properties

Excitation (nm)

498

Emission (nm)

525

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501