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5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester
5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester is an excellent amine-reactive building block that can be readily used for developing SNARF-5F pH probes. SNARF-5F has pKa of ∼7.2. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.
References
View all 11 references: Citation Explorer
Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+/H+ exchanger.
Authors: Hernández-Garduño, Sandra and Chávez, Julio C and Matamoros-Volante, Arturo and Sánchez-Guevara, Yoloxochitl and Torres, Paulina and Treviño, Claudia L and Nishigaki, Takuya
Journal: Reproduction (Cambridge, England) (2022): 125-134
Authors: Hernández-Garduño, Sandra and Chávez, Julio C and Matamoros-Volante, Arturo and Sánchez-Guevara, Yoloxochitl and Torres, Paulina and Treviño, Claudia L and Nishigaki, Takuya
Journal: Reproduction (Cambridge, England) (2022): 125-134
Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F.
Authors: Chávez, Julio C and Darszon, Alberto and Treviño, Claudia L and Nishigaki, Takuya
Journal: Frontiers in cell and developmental biology (2019): 366
Authors: Chávez, Julio C and Darszon, Alberto and Treviño, Claudia L and Nishigaki, Takuya
Journal: Frontiers in cell and developmental biology (2019): 366
In vivo quantitative imaging of tumor pH by nanosonophore assisted multispectral photoacoustic imaging.
Authors: Jo, Janggun and Lee, Chang H and Kopelman, Raoul and Wang, Xueding
Journal: Nature communications (2017): 471
Authors: Jo, Janggun and Lee, Chang H and Kopelman, Raoul and Wang, Xueding
Journal: Nature communications (2017): 471
Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis.
Authors: Roy, Jeremy W and Hill, Eric and Ruan, Ye Chun and Vedovelli, Luca and Păunescu, Teodor G and Brown, Dennis and Breton, Sylvie
Journal: American journal of physiology. Cell physiology (2013): C436-46
Authors: Roy, Jeremy W and Hill, Eric and Ruan, Ye Chun and Vedovelli, Luca and Păunescu, Teodor G and Brown, Dennis and Breton, Sylvie
Journal: American journal of physiology. Cell physiology (2013): C436-46
Pixels as ROIs (PAR): a less-biased and statistically powerful approach for gleaning functional information from image stacks.
Authors: Keller, Jacob and Homma, Kazuaki and Dallos, Peter
Journal: PloS one (2013): e69047
Authors: Keller, Jacob and Homma, Kazuaki and Dallos, Peter
Journal: PloS one (2013): e69047
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