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5-(and-6)-Carboxy SNARF-1 Succinimidyl Ester
5-(and-6)-Carboxy SNARF-1 Succinimidyl Ester is an excellent amine-reactive building block that can be used for developing SNARF-1 pH probes. It can be readily conjugated to peptides, antibodies and other amino-containing molecules such as amino-modified oligos. SNARF-1 has pKa of ∼7.5, thus extremely useful for monitoring pH changes in the range of 6.5 and 8.5. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.
References
View all 50 references: Citation Explorer
Reducing the Bitter Taste of Pharmaceuticals Using Cell-Based Identification of Bitter-Masking Compounds.
Authors: Beltrán, Leopoldo Raul and Sterneder, Sonja and Hasural, Ayse and Paetz, Susanne and Hans, Joachim and Ley, Jakob Peter and Somoza, Veronika
Journal: Pharmaceuticals (Basel, Switzerland) (2022)
Authors: Beltrán, Leopoldo Raul and Sterneder, Sonja and Hasural, Ayse and Paetz, Susanne and Hans, Joachim and Ley, Jakob Peter and Somoza, Veronika
Journal: Pharmaceuticals (Basel, Switzerland) (2022)
Ocean acidification impacts sperm swimming performance and pHi in the New Zealand sea urchin Evechinus chloroticus.
Authors: Hudson, Michael E and Sewell, Mary A
Journal: The Journal of experimental biology (2022)
Authors: Hudson, Michael E and Sewell, Mary A
Journal: The Journal of experimental biology (2022)
Durability of Implanted Low-Density Polyacrylamide Hydrogel Used as a Scaffold for Microencapsulated Molecular Probes inside Small Fish.
Authors: Shchapova, Ekaterina and Titov, Evgeniy and Gurkov, Anton and Nazarova, Anna and Borvinskaya, Ekaterina and Timofeyev, Maxim
Journal: Polymers (2022)
Authors: Shchapova, Ekaterina and Titov, Evgeniy and Gurkov, Anton and Nazarova, Anna and Borvinskaya, Ekaterina and Timofeyev, Maxim
Journal: Polymers (2022)
Calibration and measurement of mitochondrial pH in intact adult rat cardiomyocytes.
Authors: Gao, Meng and Qin, Yuan and Li, Anqi and Liu, Hanyu and Chen, Lei and Liu, Bilin and Zhang, Ying and Gao, Yufei and Gong, Guohua
Journal: STAR protocols (2021): 100543
Authors: Gao, Meng and Qin, Yuan and Li, Anqi and Liu, Hanyu and Chen, Lei and Liu, Bilin and Zhang, Ying and Gao, Yufei and Gong, Guohua
Journal: STAR protocols (2021): 100543
Studying Proton Gradients Across the Nuclear Envelope.
Authors: Martínez-Zaguilán, Raul and Sennoune, Souad R
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 47-63
Authors: Martínez-Zaguilán, Raul and Sennoune, Souad R
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 47-63
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