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5'-Amino-Modifier C6, TFA-protected
5'-Modifiers are used in DNA synthesizers to functionalize the 5'-terminus of the target oligonucleotide. 5'-amino-modifier C6 is a linker designed to be added at the terminus of an oligonucleotide synthesis to introduce a primary amino group into an oligo. This resulted amino group is suitable for immediate conjugation on the synthesis column with molecules containing an activated carboxy group, resulting in a stable amide linkage.
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of 5'-Amino-Modifier C6, TFA-protected to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 241.885 µL | 1.209 mL | 2.419 mL | 12.094 mL | 24.188 mL |
| 5 mM | 48.377 µL | 241.885 µL | 483.77 µL | 2.419 mL | 4.838 mL |
| 10 mM | 24.188 µL | 120.942 µL | 241.885 µL | 1.209 mL | 2.419 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 7 references: Citation Explorer
Cellulose/DNA Hybrid Nanomaterials
Authors: Mangalam AP, Simonsen J, Benight AS.
Journal: Biomacromolecules. (2009)
Authors: Mangalam AP, Simonsen J, Benight AS.
Journal: Biomacromolecules. (2009)
Fluorinated alcohol mediated control over cis vs trans opening of benzo[a]pyrene-7,8-diol 9,10-epoxides at C-10 by the exocyclic amino groups of O6-allyl protected deoxyguanosine and of deoxyadenosine
Authors: Yagi H, Jerina DM.
Journal: J Org Chem (2007): 6037
Authors: Yagi H, Jerina DM.
Journal: J Org Chem (2007): 6037
Rapid synthesis of DNA-cysteine conjugates for expressed protein ligation
Authors: Lovrinovic M, Niemeyer CM.
Journal: Biochem Biophys Res Commun (2005): 943
Authors: Lovrinovic M, Niemeyer CM.
Journal: Biochem Biophys Res Commun (2005): 943
High throughput purification of amino-modified oligonucleotides and its application to novel detection system of gene expression
Authors: Kojima N, Sugino M, Mikami A, Nonaka K, Fujinawa Y, Ueda Y, Sato K, Ohtsuka E, Matsubara K, Komatsu Y.
Journal: Nucleic Acids Symp Ser (Oxf) (2005): 181
Authors: Kojima N, Sugino M, Mikami A, Nonaka K, Fujinawa Y, Ueda Y, Sato K, Ohtsuka E, Matsubara K, Komatsu Y.
Journal: Nucleic Acids Symp Ser (Oxf) (2005): 181
Synthesis of covalent DNA-protein conjugates by expressed protein ligation
Authors: Lovrinovic M, Spengler M, Deutsch C, Niemeyer CM.
Journal: Mol Biosyst (2005): 64
Authors: Lovrinovic M, Spengler M, Deutsch C, Niemeyer CM.
Journal: Mol Biosyst (2005): 64
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