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LRB Red™ SE
Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of LRB Red™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 130.398 µL | 651.992 µL | 1.304 mL | 6.52 mL | 13.04 mL |
| 5 mM | 26.08 µL | 130.398 µL | 260.797 µL | 1.304 mL | 2.608 mL |
| 10 mM | 13.04 µL | 65.199 µL | 130.398 µL | 651.992 µL | 1.304 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Sunnyvale Red™ SE *Superior 6-ROX Replacement* | 591 | 624 | - | - | - |
| California Red™ SE | 592 | 609 | 1000001 | 0.24 | 0.194 |
Citations
View all 2 citations: Citation Explorer
Entry of a six-residue antimicrobial peptide derived from lactoferricin B into single vesicles and Escherichia coli cells without damaging their membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017): 4419--4431
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017): 4419--4431
Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)
References
View all 22 references: Citation Explorer
One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232
O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44
A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118
Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431
De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library
Authors: Houghten RA, Dooley CT, Appel JR.
Journal: Bioorg Med Chem Lett (2004): 1947
Authors: Houghten RA, Dooley CT, Appel JR.
Journal: Bioorg Med Chem Lett (2004): 1947
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