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Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester]
Alkyne Modifier II, NHS ester is an alkyne-containing reagent that reacts with primary amines at pH 7-9 forming a stale amide bond. The terminal alkyne group reacts with azides by a mechanism known as the Cu-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. It is widely used to introduce an alkynyl group into amino-modified oligonucleotides, peptides, proteins and other biological molecules. Compared to Alkyne Modifier I, NHS ester (4531), it adds a longer PEG4 spacer arm to the modified molecules.
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Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 249.122 µL | 1.246 mL | 2.491 mL | 12.456 mL | 24.912 mL |
| 5 mM | 49.824 µL | 249.122 µL | 498.244 µL | 2.491 mL | 4.982 mL |
| 10 mM | 24.912 µL | 124.561 µL | 249.122 µL | 1.246 mL | 2.491 mL |
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Page updated on December 17, 2024
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