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Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester]
Alkyne Modifier I, NHS ester (propargyl-NHS ester) is an alkyne-containing reagent that reacts with primary amines at pH 7-9 forming a stale amide bond. The terminal alkyne group reacts with azides by a mechanism known as the Cu-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. A short spacer arm adds minimal mass to modified molecules. It is widely used to introduce an alkynyl group into amino-modified oligonucleotides, peptides, proteins and other biological molecules.
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Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 444.05 µL | 2.22 mL | 4.44 mL | 22.202 mL | 44.405 mL |
| 5 mM | 88.81 µL | 444.05 µL | 888.099 µL | 4.44 mL | 8.881 mL |
| 10 mM | 44.405 µL | 222.025 µL | 444.05 µL | 2.22 mL | 4.44 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Citations
View all 34 citations: Citation Explorer
Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide-Alkyne Click Chemistry in Physiological Conditions
Authors: Walden, G., Liao, X., Riley, G., Donell, S., Raxworthy, M. J., Saeed, A.
Journal: Bioconjug Chem (2019): 531-535
Authors: Walden, G., Liao, X., Riley, G., Donell, S., Raxworthy, M. J., Saeed, A.
Journal: Bioconjug Chem (2019): 531-535
Ultrasound-assisted Cu(I)-catalyzed azide-alkyne click cycloaddition as polymer-analogous transformation in chitosan chemistry. High antibacterial and transfection activity of novel triazol betaine chitosan derivatives and their nanoparticles
Authors: Kritchenkov, A. S., Egorov, A. R., Dysin, A. P., Volkova, O. V., Zabodalova, L. A., Suchkova, E. P., Kurliuk, A. V., Shakola, T. V.
Journal: Int J Biol Macromol (2019): 592-603
Authors: Kritchenkov, A. S., Egorov, A. R., Dysin, A. P., Volkova, O. V., Zabodalova, L. A., Suchkova, E. P., Kurliuk, A. V., Shakola, T. V.
Journal: Int J Biol Macromol (2019): 592-603
Injectable Catalyst-Free Poly(Propylene Fumarate) System Cross-Linked by Strain Promoted Alkyne-Azide Cycloaddition Click Chemistry for Spine Defect Filling
Authors: Liu, X., Miller, A. L., 2nd, Xu, H., Waletzki, B. E., Lu, L.
Journal: Biomacromolecules (2019): 3352-3365
Authors: Liu, X., Miller, A. L., 2nd, Xu, H., Waletzki, B. E., Lu, L.
Journal: Biomacromolecules (2019): 3352-3365
CuAAC click chemistry for the enhanced detection of novel alkyne-based natural product toxins
Authors: Hems, E. S., Wagstaff, B. A., Saalbach, G., Field, R. A.
Journal: Chem Commun (Camb) (2018): 12234-12237
Authors: Hems, E. S., Wagstaff, B. A., Saalbach, G., Field, R. A.
Journal: Chem Commun (Camb) (2018): 12234-12237
Ribosome-Templated Azide-Alkyne Cycloadditions Using Resistant Bacteria as Reaction Vessels: in Cellulo Click Chemistry
Authors: Jin, X., Daher, S. S., Lee, M., Buttaro, B., Andrade, R. B.
Journal: ACS Med Chem Lett (2018): 907-911
Authors: Jin, X., Daher, S. S., Lee, M., Buttaro, B., Andrade, R. B.
Journal: ACS Med Chem Lett (2018): 907-911
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