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4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4*
4-Methylumbelliferyl-beta-D-glucoside is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 295.578 µL | 1.478 mL | 2.956 mL | 14.779 mL | 29.558 mL |
| 5 mM | 59.116 µL | 295.578 µL | 591.156 µL | 2.956 mL | 5.912 mL |
| 10 mM | 29.558 µL | 147.789 µL | 295.578 µL | 1.478 mL | 2.956 mL |
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References
View all 5 references: Citation Explorer
Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors
Authors: Wrodnigg TM, Withers SG, Stutz AE.
Journal: Bioorg Med Chem Lett (2001): 1063
Authors: Wrodnigg TM, Withers SG, Stutz AE.
Journal: Bioorg Med Chem Lett (2001): 1063
3-Hydroxycoumarins: first direct preparation from coumarins using a Cu2(+)-ascorbic acid-O2 system, and their potent bioactivities
Authors: Aihara K, Higuchi T, Hirobe M.
Journal: Biochem Biophys Res Commun (1990): 169
Authors: Aihara K, Higuchi T, Hirobe M.
Journal: Biochem Biophys Res Commun (1990): 169
Subcellular Localization of 2-(beta-d-Glucosyloxy)-Cinnamic Acids and the Related beta-glucosidase in Leaves of Melilotus alba Desr
Authors: Oba K, Conn EE, Canut H, Boudet AM.
Journal: Plant Physiol (1981): 1359
Authors: Oba K, Conn EE, Canut H, Boudet AM.
Journal: Plant Physiol (1981): 1359
Effect of coumarin on growth and metabolism of Pythium
Authors: Neto FC, Dietrich SM.
Journal: Appl Environ Microbiol (1977): 258
Authors: Neto FC, Dietrich SM.
Journal: Appl Environ Microbiol (1977): 258
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