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DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8*
An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 253.588 µL | 1.268 mL | 2.536 mL | 12.679 mL | 25.359 mL |
| 5 mM | 50.718 µL | 253.588 µL | 507.177 µL | 2.536 mL | 5.072 mL |
| 10 mM | 25.359 µL | 126.794 µL | 253.588 µL | 1.268 mL | 2.536 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 124 references: Citation Explorer
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Evaluation of an imaging platform during the development of a FRET protease assay
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
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