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NTA succinimidyl ester
Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) is such a ligand commonly used in commercially available resins for purifying proteins. On the other hand, tag-NTA conjugates are used for detecting polyhistidine-tagged proteins. NTA succinimidyl ester is an excellent building block that can be readily used to attach NTA groups to a biological substrate or a surface that has amino groups. Through the NTA moiety attached to the substrate or surface, genetically expressed proteins bearing the hexahistidine extension (HIS Tag) can be detected or immobilized via Ni-mediated His-Tag method.
Citations
View all 1 citations: Citation Explorer
Transition Metal Ion FRET-Based Probe to Study Cu (II)-Mediated Amyloid-$\beta$ Ligand Binding
Authors: Wu, Ri and Svingou, Despoina and Metternich, Jonas B and Benzenberg, Lukas R and Zenobi, Renato
Journal: Journal of the American Chemical Society (2024)
Authors: Wu, Ri and Svingou, Despoina and Metternich, Jonas B and Benzenberg, Lukas R and Zenobi, Renato
Journal: Journal of the American Chemical Society (2024)
References
View all 31 references: Citation Explorer
Proteomics analysis of host cell proteins after immobilized metal affinity chromatography: Influence of ligand and metal ions.
Authors: Lingg, Nico and Öhlknecht, Christoph and Fischer, Andreas and Mozgovicz, Markus and Scharl, Theresa and Oostenbrink, Chris and Jungbauer, Alois
Journal: Journal of chromatography. A (2020): 461649
Authors: Lingg, Nico and Öhlknecht, Christoph and Fischer, Andreas and Mozgovicz, Markus and Scharl, Theresa and Oostenbrink, Chris and Jungbauer, Alois
Journal: Journal of chromatography. A (2020): 461649
Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns.
Authors: Liu, Guizhen and Fu, Tao and Han, Ying and Hu, Shichen and Zhang, Xuepei and Zheng, Mengmeng and Hao, Piliang and Pan, Lifeng and Kang, Jingwu
Journal: Analytical chemistry (2020): 3913-3922
Authors: Liu, Guizhen and Fu, Tao and Han, Ying and Hu, Shichen and Zhang, Xuepei and Zheng, Mengmeng and Hao, Piliang and Pan, Lifeng and Kang, Jingwu
Journal: Analytical chemistry (2020): 3913-3922
Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A case study with a Ni2+-tacn-based IMAC system.
Authors: Yang, Yuanzhong and Mitri, Khosse and Zhang, Chunfang and Boysen, Reinhard I and Hearn, Milton T W
Journal: Protein expression and purification (2019): 51-61
Authors: Yang, Yuanzhong and Mitri, Khosse and Zhang, Chunfang and Boysen, Reinhard I and Hearn, Milton T W
Journal: Protein expression and purification (2019): 51-61
Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue.
Authors: Kokhan, Oleksandr and Marzolf, Daniel R
Journal: Analytical biochemistry (2019): 113347
Authors: Kokhan, Oleksandr and Marzolf, Daniel R
Journal: Analytical biochemistry (2019): 113347
Complementary IMAC enrichment methods for HLA-associated phosphopeptide identification by mass spectrometry.
Authors: Abelin, Jennifer G and Trantham, Paisley D and Penny, Sarah A and Patterson, Andrea M and Ward, Stephen T and Hildebrand, William H and Cobbold, Mark and Bai, Dina L and Shabanowitz, Jeffrey and Hunt, Donald F
Journal: Nature protocols (2015): 1308-18
Authors: Abelin, Jennifer G and Trantham, Paisley D and Penny, Sarah A and Patterson, Andrea M and Ward, Stephen T and Hildebrand, William H and Cobbold, Mark and Bai, Dina L and Shabanowitz, Jeffrey and Hunt, Donald F
Journal: Nature protocols (2015): 1308-18
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