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5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*
TAMRA is one of the most popular fluorophores used in various bioconjugations. TMR is a bright orange fluorophore. 5(6)-TAMRA is the mixture of two carboxy tetramethylrhodamine (TMR) isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings. It can be readily convert to the amine-reactive 5(6)-TAMRA, SE.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 232.315 µL | 1.162 mL | 2.323 mL | 11.616 mL | 23.232 mL |
| 5 mM | 46.463 µL | 232.315 µL | 464.63 µL | 2.323 mL | 4.646 mL |
| 10 mM | 23.232 µL | 116.158 µL | 232.315 µL | 1.162 mL | 2.323 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] | 522 | 546 | 94000 | 0.24 | 0.214 |
| 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* | 578 | 604 | 82000 | - | 0.168 |
| 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* | 544 | 570 | 100000 | 0.27 | 0.34 |
| 6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5-TAMRA cadaverine | 552 | 578 | 90000 | 0.32 | 0.178 |
Show More (8) | |||||
Citations
View all 8 citations: Citation Explorer
Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide
Authors: Hakata, Yoshiyuki and Yamashita, Kazuma and Hashimoto, Sonoko and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Pharmaceutics (2023): 1048
Authors: Hakata, Yoshiyuki and Yamashita, Kazuma and Hashimoto, Sonoko and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Pharmaceutics (2023): 1048
Complementary leucine zippering system for effective intracellular delivery of proteins by cell-penetrating peptides
Authors: Kitamatsu, Mizuki and Yuasa, Hiroki and Ohtsuki, Takashi and Michiue, Hiroyuki
Journal: Bioorganic \& Medicinal Chemistry (2021): 116036
Authors: Kitamatsu, Mizuki and Yuasa, Hiroki and Ohtsuki, Takashi and Michiue, Hiroyuki
Journal: Bioorganic \& Medicinal Chemistry (2021): 116036
Intracellular delivery of a peptide nucleic acid-based hybrid of an autophagy inducing peptide with a cell-penetrating peptide
Authors: Hakata, Yoshiyuki and Ishikawa, Suzuka and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Organic \& Biomolecular Chemistry (2020): 1978--1986
Authors: Hakata, Yoshiyuki and Ishikawa, Suzuka and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Organic \& Biomolecular Chemistry (2020): 1978--1986
CCR2 signal facilitates thymic egress by priming thymocyte responses to sphingosine-1-phosphate
Authors: Aili, Abudureyimujiang and Zhang, Jie and Wu, Jia and Wu, Haoming and Sun, Xiuyuan and He, Qihua and Jin, Rong and Zhang, Yu
Journal: Frontiers in immunology (2018): 1263
Authors: Aili, Abudureyimujiang and Zhang, Jie and Wu, Jia and Wu, Haoming and Sun, Xiuyuan and He, Qihua and Jin, Rong and Zhang, Yu
Journal: Frontiers in immunology (2018): 1263
Amikacin liposome inhalation suspension (ALIS) penetrates non-tuberculous mycobacterial biofilms and enhances amikacin uptake into macrophages
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)
References
View all 96 references: Citation Explorer
A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
Authors: Nakayama GR, Bingham P, Tan D, Maegley KA.
Journal: Anal Biochem (2006): 260
Authors: Nakayama GR, Bingham P, Tan D, Maegley KA.
Journal: Anal Biochem (2006): 260
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
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